One study utilized IP injections to study pain in the abdomen after a hysterectomy when administering anesthetic continuously vs patient-controlled. In addition, IP showed more intense Ki-67 stain in bottom of the crypt, which suggested more active mucosa repair proliferation (Fig. 6A). Secondly, as mentioned above, there are plenty of immune cells in peritoneal cavity28, which are indispensable component of MSCs aggregates. Method. Disease recovery was evaluated by histological server score, gross body weight and survival rate. Interestingly, in some IP injection mice, GFP+ cells were observed in the epithelium of the inflamed colon. Insert the needle at a shallow angle through the . Indeed, Mariana et al. Comparative analysis of chemokine receptors expression in mesenchymal stem cells derived from human bone marrow and adipose tissue. This finding was consistent with previous published paper23, in which the intraperitoneal MSCs migrating and engraft at inflamed colon were identified by Tc-99m SPECT imaging in TNBS induced colitis. Use " or 1" needles to avoid penetrating abdominal organs. Patients should be anesthetized. n=68 mice/group; n=3 for the control and naive (health mice) group. Augello, A., Tasso, R., Negrini, S. M., Cancedda, R. & Pennesi, G. Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis. ADS some potent chemo and radio-labeled drugs)59. There is a little fluid in between the peritoneal lining and your organs. Peyrin-Biroulet, L., Loftus, E. V. Jr., Colombel, J. F. & Sandborn, W. J. . Intraperitoneal Route of Drug Administration: Should it Be Used in This study included unilateral SSNHL patients who were hospitalized in our department between . Here, we also compare the expression of FoxP3, which is frequently used to quantitate Treg cells. The .gov means its official. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Comparison of colonic proliferation 7 days after MSCs injection. ***P<0.001; **P<0.01. Here, three different MSCs delivery routes: intraperitoneal (IP), intravenous (IV) and anal injection (AI) were compared on DSS-induced colitic mice model. However, is IV injection the best way for MSCs delivery in IBD? Intraperitoneal Injection in the Mouse - Research Animal Training Karp, J. M. & Leng Teo, G. S. Mesenchymal stem cell homing: the devil is in the details. The Ki-67+ cells were greatly increased after MSCs treatment. During the observation, the body weight changes and fecal blood and their survival date was faithfully record. & Prockop, D. J. Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-kappaB signaling in resident macrophages. Inactivating IL34 promotes regenerating muscle stem cell expansion and The article overviews whether IP is the best option for euthanization based on evidence associated with welfare implications. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. There are few accounts of the use of intraperitoneal injections prior to 1970. Article In mouse, the intraperitoneal and subcutaneous LD 50 values are 138 and 650 mg kg 1, respectively. Furthermore, the body weight changes displayed the less weight loss and quick weight gain of IP injection group and the maximum group difference among was reached on day 3 (Fig. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. This concern arises because MSCs has an estimated diameter of 2030m15,16. Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. Not only in animal studies, some clinical trials using MSCs to treat osteogensis imperfect (OI) in GvHD, also showed less than 1% cell were detected in target organ18,19,20. The expression level of TNF-, the most important pro-inflammatory cytokine, was markedly decreased in IP group, compared with AI (P=0.0174), IV (P=0.0019) and control group (P<0.001), respectively. Drug Deliv. Cell and tissue research 347, 419427, doi: 10.1007/s00441-011-1306-3 (2012). Normal mouse IgG was used as a substitute for the primary antibody as negative control. Subcutaneous Compared with Intraperitoneal Ketamine-Xylazine for Intraosseous. Makhija, S. et al. Collagen deposition limits immune reconstitution in the gut. Select search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources Intraperitoneal Injection Is Not a Suitable Administration Route for For some chemotherapy, such as ovarian cancer, IP injection is used to attempt to localize the drug . Article Human mesenchymal stem cells license adult CD34+ hemopoietic progenitor cells to differentiate into regulatory dendritic cells through activation of the Notch pathway. However, they found the migrated PCs was very few and these may attribute to: 1) the recipient are heathy rat. Course of Crohns disease after allogeneic marrow transplantation. Stem Cells 29, 15721579, doi: 10.1002/stem.708 (2011). The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Some of the examples depict situations where IP injections are not ideal, while others prove the advantageous uses if this delivery method. PubMed Intraperitoneal injection of magnetic Fe3O4-nanoparticle induces Berberich, S., Forster, R. & Pabst, O. Federal law restricts this drug to use by or on the order of a licensed veterinarian. Side effects. Stem cells international 2013, 732742, doi: 10.1155/2013/732742 (2013). Human. While the DiR labeled cells showed very different cell distribution. Endoscopic fluorescence imaging experiments were performed at 1-h postinjection of FA-NPs, celecoxib/FA-NPs or FNC-NPs using a Karl Storz-Endoskope System. High Pressure Nebulization (PIPAC) Versus Injection for the Intraperitoneal Administration of mRNA Complexes. Dig Dis 28, 386394, doi: 10.1159/000320392 (2010). Study showed that genetically modified MSCs to increase CXCR-4 expression will lead to an increase of MSCs migration to intestinal with radiation enteritis and then resulted in disease improvement24. Currently, two types of stem are used for the treatment of IBD, hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs). The optimum route of administration is an importance clinical issue not only for chemical drug, but also for stem cytotherapy. PDF UBC ANIMAL CARE COMMITTEE TECH 10a Intraperitoneal Injection in the After 3 passages, spindle-shaped cell colonies gradually predominate in the cultures. Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? This is really an interesting finding, however exact mechanisms are still unknown. Article One study attempted to diagnose hepatic hydrothorax with the use of injecting Sonazoid intraperitoneally. Inflammatory bowel diseases (IBD) is a broad term that involves chronic inflammation of all or part of the gastrointestinal tract. Article The serum concentration of TNFinduced protein 6 (TSG-6) was measured in DSS colitic mice at 48 and 72hours after different routes of MSCs administrations. Subcutaneous. Zhu, H. et al. IP injections were suitable for serial imaging as well as imaging mice at multiple time points, which would be ideal for longitudinal drug treatment experiments. Google Scholar. Though genetic and environmental factors are believed to be involved in the disease, not much progress has been made on therapy. Intraperitoneal chemotherapy for peritoneal metastases: an expert opinion. Macrophage Reverse Cholesterol Transport | Circulation By using this techniques, the catheter complications, in particular obstruction, bowel and vaginal cuff perforation were greatly reduced61. [1] Fluids are injected intraperitoneally in infants, also used for peritoneal dialysis. The bilateral femurs and tibias were aseptically excised, stripped of connective tissues and then stored in PBS. In order to compare the difference of MSCs colonization among the three delivery routes, a nontoxic NIR tracer DiR was introduced to label MSCs. Interferon-gamma (IFN-gamma)- and tumour necrosis factor (TNF)-induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium (DSS)-induced colitis in mice. Our findings suggested that IP delivery showed higher MSCs and better experimental colitis recovery, might be an ideal way for MSCs therapy in IBD. The problem is obvious, the solution is not: numbers do matter in cardiac cell therapy! The following approaches are novel, and require further testing in humans before being accepted as potential treatment options: Such huge need of MSCs poses a cell source issue, which is a continuing technically and operationally challenge, since most of human ongoing clinical trials use significant less cell dose12. Gastroenterology 145, 158165 e152, doi: 10.1053/j.gastro.2013.04.007 (2013). Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? For this reason, more MSCs aggregates would form for IP route and produce higher level of serum TSG-6 under TNF- activation, which help reducing the colon inflammation. And the loss of MSCs in the colon may attribute to many reasons, such as, wash out, cell death, or even rejection via the innate immune system. Caplan, A. I. More recently the IP route has been used for chemotherapy in patients with intra-abdominal malignancies, i.e. Injected dissociative agent combinations. Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. All reactions were performed in triplicated. Comparison of colonic FoxP3 expression 7 days after MSCs injection. Kenny et al. Ankrum, J. Samples were prepared and tested as previously reported47. Acute colitis was induced in C57BL/6 male mice by feeding 5% DSS (molecular weight 40000Da; Sigma) dissolved water for 5 days, followed by five days of regular drinking water according to our previously published study63. These early uses of Intraperitoneal injections provide good examples of how the delivery method can be used, and provides a base for future studies on how to properly inject mice for research. Comparison of the Effects of Intraperitoneal Injection with Carbon Muller-Ehmsen, J. Would you like email updates of new search results? Acceptable Method (s) Injected barbiturates and barbiturate combinations. Intraperitoneal drug therapy: an advantage - PubMed and transmitted securely. Growth hormone Best way for mesenchymal stem cells transplantation for colitis. [10] The results depicted that ketobemidone consumption was significantly lower when patients controlled anesthetic through IP. Fresh feces from animals were collected for occult blood test, using fecal occult blood kit (Baso Diagnostics Inc., Zhuhai, China) according to the manufactures recommendations. However, more studies described MSCs as hit and run therapy, which discovered most of MSCs died and cleared from the body within 48 to 72h20,41,42,43. Krampera, M. et al. 81370585, No. Thus, IP and AI routes are free of pulmonary embolism issue. It also shows how easily substances are absorbed through the peritoneal cavity after an IP injection. Free DiR and healthy mice (without colitis) were served as control. Cardiovascular research 96, 208209; discussion 210203, doi: 10.1093/cvr/cvs202 (2012). National Cancer Institute. Ray, A. The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. The fecal occult blood test on the day 3 also showed nearly complete absence of occult blood in IP group. HHS Vulnerability Disclosure, Help An improved mouse model of sepsis based on intraperitoneal injections of the enriched culture of cecum slurry . Nature medicine 5, 309313, doi: 10.1038/6529 (1999). The IP and AI groups showed mean 50.4% and 46.7% Ki-67+ cells/crypt respectively. PubMed Rodents. : 1950) 180, 15981608 (2008). Understanding when intraperitoneal injections can be utilized and in what applications is beneficial to advance current drug delivery methods and provide avenues for further research. Maximum 15 10 slow injection 10 2 (divided in 2-3 sites) 0.5 (Max 2 ml limit) Minipig Ideal 10 1-5 bolus 1 1 0.25 Maximum 15 10 slow injection 20 3 (divided in 2-3 sites) 0.5 (Max 5 ml limit per site) * Bolus injections are typically dosed in less than 1 minute. Other gap junction blockers were also occasionally reported to . B.X. PDF Guidelines for the Research Use of Adjuvants - National Institutes of Integrative genetic analysis identifies FLVCR1 as a plasma-membrane Generally, the mice were killed by cervical dislocation. PubMed Inflammatory bowel disease (IBD). These complications include catheter infection, physical damages to intra-abdominal structures, such as vaginal vault perforation, bladder erosion and bowel perforation58 and direct drug toxicity to non-specific abdomen organ (e.g.
